Proteomic Dataset of Healthy Hamlin Sweet Orange (Citrus sinensis) Phloem Sap

This dataset contains proteomic data generated from phloem sap collected from healthy ‘Hamlin’ sweet orange (Citrus sinensis) plants. Phloem sap was collected from greenhouse-grown plants grafted onto UFR-17 rootstock using a centrifugation-based collection method. Three biological replicates were analyzed by liquid chromatography–tandem mass spectrometry (LC–MS/MS) using a Bruker timsTOF fleX mass spectrometer. Proteomic data were processed and searched using Mascot and Scaffold DDA against the Citrus sinensis cv. Valencia genome v2.0 reference proteome. The dataset includes mass spectrometry-derived proteomic results and associated protein identification and annotation files used to characterize the phloem-sap proteome. The dataset supports the characterization of the healthy citrus phloem-sap proteome and provides a reference resource for future studies of citrus physiology, phloem biology, protein secretion, and interactions with Huanglongbing-associated pathogens and other stress conditions.