Leishmania major raw data of all the transcript isoforms generated with PacBio

Total RNA samples from three biological replicates (GC1003378, GC1003379,GC1003380) of Leishmania major (Friedlin strain) promastigotes were separately retrotranscribed using poly-T oligonucleotide into cDNA. After a step of PCR amplification, the resulting DNA was sequenced using the Single Molecule, Real-Time (SMRT) Sequencing methodology (PacBio) by the sequencer: 1M V3.0 Long Read SMRTCell and chemistry. High quality reads were used to generate circular consensus sequences (CCS), which were classified as complete or truncated transcripts, depending on the presence at their 5'-end of the mini-exon (spliced-leader) sequence or not, respectively. Here, we are submitting both the raw data and the sequences of all the transcript isoforms generated in this study.