These are H. pylori cells that don't have a complete FliP. In addition, FliO was knocked out in . these cells. The aim is to study the assembly of the flagellar motor and the core complex.
JV4404 is deletion of the Dot.Icm system. Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in