ChIP-seq analysis of androgen receptor binding in epididymis of ArKI mice. ChIP-seq analysis of androgen receptor binding in epididymis of ArKI mice

To analyze how SUMOylation of AR affects its chromatin binding, we generated a mouse model (ArKI) in which the two conserved SUMO acceptor lysines of androgen receptor (AR) were permanently abolished by converting them to arginines (ArK381R, K500R). ChIP-seq was used to analyse how the lack of AR SUMOylation affects the chromatin binding of AR in epididymis. Overall design: For each sample, caput epididymides (containing initial segments) from three wild type (WT) or ArKI (KI) mice were combined, chromatin binding of AR was analyzed using ChIP and sequenced using Illumina HiSeq 3000 system. We generated two replicates from WT and ArKI mice.