Glutamine synthetase gene glnA plays an important role in curdlan biosynthesis of Agrobacterium sp. CGMCC 11546

Purpose： The goal of this study is compare the effect of glnA gene in curdlan synthesis in Agrobacterium sp. CGMCC 11546. methods: The transcriptional and metabolomics analysis the function of glnA in Agrobacterium sp. CGMCC 11546. Results: The transcriptional and metabolomics showed that the decrease of curdlan production in the ΔglnA mutants may be caused by the insufficient supply of energy ATP conclusion: glnA play an important role in curdlan synthesis in Agrobacterium sp. CGMCC 11546 The total RNA of each sample from the wild type strain and the gene deletion mutants after 24 hours fermentation, samples from glnA deletion mutant named glnA-1, glnA-2 and glnA-3; three biological repeats. samples from the wild type strain named WT-1, WT-2 and WT-3; three biological repeats