Text Aberration Summary Reports of 77 MDS patients.

Aim - About 40% - 50% of patients with myelodysplastic syndromes (MDS) present with a normal karyotype and develop different courses of disease. To advance the biological understanding and to find molecular prognostic markers, we performed a high-resolution CGH + SNP array along with NGS of 77 MDS patients and they were clinically followed up.Methodology - Chromosomal microarray analysis was performed on the Agilent’s SureScan microarray scanner using SurePrint G3 Human CGH+SNP, 4x180K array. This microarray consists of approximately 180000 oligonucleotide + SNP probes. These probes allow detection of deletions above 100 Kb and duplication above 200 kb in entire human genome. High quality genomic DNA was extracted and labeled enzymatically with Cy3 or Cy5 dyes along with reference DNA. Purified labeled sample were hybridized to the SurePrint G3 Human aCGH+SNP 4x180K array slide. Data was analysed using CytoGenomics 5.1.2.1 with validated parameters on in-house samples with known CNVs. Text Aberration Summary Reports were produced using CGH module of Agilent Genomic Workbench that helped examine and analyze results. Each (n=77) text Aberration Summary Report includes header information that contains the parameters used for the data pre-processing and analysis.