Spine patterning is guided by segmentation of the notochord sheath

Notochord sheath cells were FACS isolated from 13 days post fertilization (dpf) transgenic zebrafish. Three biological replicates were used for each population, and expression profiles were determined using Illumina HiSeq. Comparison of the sample groups allowed for identification of unique candidates. The sequence reads that passed quality filters were analyzed using HISAT2, and gene counts were analyzed using HTSeq. 3 distinct samples, with 3 replicates each. 3 distinct samples: col9a2+, entpd5a+, and col9a2/entpd5a+ (double) were isolated from the notochord sheath cells of 13 dpf transgenic zebrafish.