Transcriptome analysis of lung epithelial cells from various developmental stages (E13.5, E15.5, E18.5, P14, and P56)

We investigated whether cell transplantation potential differs between the different developmental stages of the lungs. We found that epithelial cells derived from different developmental stages show different engraftment potential. To identify transcriptional signatures of epithelial cells that are associated with the phenomenon, we performed a time series transcriptome analysis of epithelial cells of the developing lungs. Overall design: Lung epithelial cells from various developmental stages (E13.5, E15.5, E18.5, P14, and P56) were purified by cell sorting. The time series transcriptome of the epithelial cells was generated by 3'SAGE-seq using Ion Proton sequencer.