Genome-wide profiling of H3K4me3 and H3K27me3 in rice male meiocytes using low cell number ChIP-seq

Meiotic cell-specific chromatin profiling at the gene level is still not fully elucidated, which is largely due to techniques challenges for collection of enough synchronized cells of interest for conventional chromatin immunoprecipitation. This leaves the open question in a state of incomprehension, which is about how chromatin reprogramming links to transcriptional dynamics at individual well-defined meiotic cell-type or stage. To address this, we developed a low cell number ChIP-seq (LCN ChIP-seq) approach by using 8-10K cells, which was for the first time applied for profiling of an active mark H3K4me3 and a repressive mark H3K27me3 genome-wide for male meiocytes at prophase I stage. ChIP-seq