We develop a universal method to label individual DNA molecules for analyzing diverse types of rare genetic variants, with frequency as low as 4x10-5, using short- or long-read sequencing. It enables
We created a mutator protein, eMutaT7[transition] with targeted in vivo hypermutation in E. coli. To investigate the gene-specific mutagenesis, we sequenced ~3.3 kb DNA around the target gene from cel
NGS data for primary WT as wells as primary and immortalized RDEB keratinocytes treated with frameshift CRISPR gRNAs. gRNA with precise and predictable repair outcome (+1 nucleotide insertion).
