Localization of Cst26p in <i>Δcst26</i> cells transformed with GFP-tagged Cst26 and LPAAT activity assay.

(A) Micrographs of ΔCST26 cells transformed with the empty vector. DIC, differential interference contrast. (B) flurescence microscopy ofΔCST26 cells transformed with GFP-tagged Cst26. (C) PI 34:1 and PI36:1 levels were restored in the CST26-transformed ΔCST26 mutant; (D) In the LPAAT activity assay, CST26 shows acyltransferase activity toward 18:0, 17:0, 16:0, 14:0 (blue rectangles) with 16.7% of PA17:0/18:0, 65.1% of PA17:0/17:0, 28.5% of PA17:0/16:0 and 65.8% of PA 17:0/14:0 when compared with corresponding PA in wild type, whereas SLC1 shows activity toward 10:0, 12:0, 14:0 and 18:1 (red rectangles) with 29% of PA17:0/10:0, 19% of PA17:0/12:0, 52% of PA17:0/14:0 and 76% of PA17:0/18:1 when compared with corresponding PA in wild type. Levels of individual PA species were expressed as normalized intensity to internal standard PA14:0/14:0.