Resistance of microbial community in activated sludge to nano-Ag stress through regulation of AHLs-mediated quorum sensing

metatranscriptomics. The molecular mechanism of quorum sensing how to regulate the changes in microbial functional gene activity and key metabolites to respond to nano-Ag stress remains elusive.We combined the long-term bioreactor with the metagenomics, metatranscriptomics, and metabolomics to explore the specific mechanism, which involved three dimensions: species composition, gene expression, and metabolic pathway.