Transriptome profiling of mutant p53 skin deficient in AURORA-A KINASE

We performed next-generation sequencing of RNA to determine the transcriptional changes in hyperplastic mutant p53 skin after the epithelial deletion of the AURORA-A Kinase gene in mice. Overall design: In this study skin RNA of four experimental groups were sequenced. Mice with the p53f, p53lslR172H, and KrasLSLG12D knock in alleles were crossed with the K14CreER(T) transgene to generate Loss-Of-Function (LOF) p53 mice (p53f/lslR172H;KraslslG12D;K14CreER) or GOF p53 mice (p53f/lslR172H;KraslslG12D;K14CreER). A floxed Aur-A allele was introduced to GOF or LOF p53 mice to generate Aur-Af/f; GOF or Aur-Af/f; LOF p53 mice. Tamoxifen was given to 8-10wk old mice in the diet for 14 d to induce recombination of floxed alleles. The mice were also co-treated with topical 12-O-tetradecanoylphorbol-13-acetate for 7d. Skin samples were harvested at the end of the study period.