Methods and compositions for improving genetically engineered microorganisms

The present invention provides a bacterial composition comprising: one or more genetically engineered bacterial strains immobilizing atmospheric nitrogen in an agricultural system， wherein the bacterial strains are bcsII， bcsIII， yjbE， fhaB， pehA， glgA， otsB， treZ And one or more genes selected from the group consisting of cysZ. The present invention further provides a bacterial composition and method for increasing colonization of plant growth promoting bacterial strains in plants， wherein the plant growth promoting bacterial strains have been remodeled to increase colonization of the plants. In another aspect， the present invention provides a method of increasing nitrogen or nitrogen immobilization available to plants. The present disclosure provides a bacterial composition， comprising: at least one genetically engineered bacterial strain that fixes atmospheric nitrogen in an agricultural system， wherein the bacterial strain comprises a modification in or one or more genes selected from the group consisting of bcsll， bcslll， yjbE， fhaB， pehA， glgA， otsB， treZ， and cysZ. The present disclosure further provides a bacterial composition and method for increasing the colonization of a plant growth promoting bacterial strain on a plant， wherein the plant growth promoting bacterial strain has been remodeled to increase colonization of said plant， In a further aspect， the present disclosure provides methods of increasing nitrogen or nitrogen fixation available to a plant. The present disclosure provides a bacterial composition， comprising: at least one genetically engineered bacterial strain that fixes atmospheric nitrogen in an agricultural system， wherein the bacterial strain comprises a modification in or one or more genes selected from the group consisting of bcsll， bcslll， yjbE， fhaB， pehA， glgA， otsB， treZ， and cysZ. The present disclosure further provides a bacterial composition and method for increasing the colonization of a plant growth promoting bacterial strain on a plant， wherein the plant growth promoting bacterial strain has been remodeled to increase colonization of said plant， In a further aspect， the present disclosure provides methods of increasing nitrogen or nitrogen fixation available to a plant.