Chemotactic response of hESCs to trophoblast secretory products identified by proteome profiling.

(A) Decidualized hESCs were analyzed in transwell migration assay in response to PDGF-BB, HB-EGF, TCM, PDGF-AA, PLGF-1 or VEGF-165. Motility indices are shown as means±SD (n = 3), and were analyzed by ANOVA and Dunnett test. ***, P<0.001 compared to the control without chemoattractant. (B, C) Effect of neutralization of PDGF activity. Decidualized hESCs were subjected to transwell migration assay with two different doses of PDGF-AA (B) or with TCM and two individual VECM preparations (C) in the absence or presence of a neutralizing antibody to PDGF-AA/-AB/-BB (pan). Motility indices are shown as means±SD (n = 3) and were analyzed by ANOVA and Dunnett or Tukey test. ***, P<0.001; **, P<0.01 in the absence vs. presence of antibody. a, P<0.001; b, P<0.01; c, P<0.05 compared to the respective control without stimulation or antibody (white or light grey columns).