RNA-Seq of lung tissue from WT or interleukin-1alpha nuclear localisation sequence mutant mice infected with influenza A

We generated a mouse with a mutated IL-1alpha nuclear localisation sequence, causing loss of pro-IL-1alpha nuclear localisation. We tested whether this loss of nuclear pro-IL-1alpha affected the response of these mice to Influenza A infection. WT or mNLS mice were anaesthetized by 2.5% inhalation isoflurane and intranasally infected with 10^3 PFU of the IAV strain X31 (H3N2), in a volume of 30 µL PBS, on day 0. Infected WT and mNLS mice, and heterozygous control uninfected mice, were culled on day 7 post-IAV X31 infection, and the lung RNA was extracted.