The thermodynamic contributions to duplex formation of all 32 possible single-nucleotide dangling ends on a Watson–Crick pair are reported. In most instances, dangling ends are stabilizing with free e
'Stacking hybridization reactions' wherein two or more short DNA oligomers hybridize in a contiguous tandem orientation onto a longer complementary DNA single strand have been employed to enhance a va
The melting temperature and thermodynamic parameters derived from thermal denaturation for the formation of duplex DNA with and without drug binding at 25°C.
Formamide lowers melting temperatures (Tm) of DNAs linearly by 2.4-2.9 degrees C/mole of formamide (C(F)) depending on the (G+C) composition, helix conformation and state of hydration. The inherent co
