Effects of PCOS faecal microbiota transplantation on the disruption of insulin sensitivity in PCOS model mice

PCOS model mice transplanted with stool suspensions from healthy controls and women with PCOS were defined as the LET+Control FMT and LET+PCOS FMT groups, respectively. After treatment with an antibiotic cocktail (20 mg/ml vancomycin, 40 mg/ml neomycin sulfate, 40 mg/ml metronidazole, 40 mg/ml ampicillin intragastrically once daily) for 5 d, the mice were orally gavaged with LET for 5 weeks and stool suspensions once a day for 1 week and once every three days for 9 weeks (LET+Control FMT: 9; LET+PCOS FMT: 9). (A) Schematic representation of the experimental design. (B) Fasting blood glucose. (C) Fasting insulin. (D) HOMA-IR. For B-D, P values were determined by two-tailed Student’s t-test, and P < 0.05 was considered statistically significant.