Clostridium difficile Biofilm with and without deoxycholate at 48H. Clostridioides difficile

To understand the molecular mechanisms induced by deoxycholate (DCA) during biofilm formation and to identify factors implicated in the strong density of biofilms in this condition, we performed a transcriptomic analysis using microarrays. The microarrays were used to compare C. difficile transcription profiles of a 48 h biofilm (in BHISG medium) treated with DCA with an untreated biofilm. Overall design: Two-conditions experiments, 630E strain WT with and without Deoxycholate Biofilm 48h, 4 biological replicates for each condition