five

data sharing.xlsx

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NIAID Data Ecosystem2026-03-13 收录
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https://figshare.com/articles/dataset/data_sharing_xlsx/20301495
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This is the summary data used for qPCR graph making.   The geometric mean of mRNA expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and actin beta (β-actin) has been used as housekeeping genes confirmed by their consistent Ct values among the treatments (P > 0.1).     Samples were run in triplicate, and relative gene expression data were analyzed using the 2-ΔΔCt. The mean ΔCt of each marker gene from the SO group was used to calculate the ΔΔCt value, and 2-ΔΔCt expression levels were normalized to 1 for the SO group and the FO group expression level presented as fold change.
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2022-08-04
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