Gene expression profile at single cell level of isofortex from Control and AS3MTd2d3-Tg mice

Cell-type specific changes of isocortex in schizophrenia patients play critical roles in perception, cognition, emotion, and learning. We used single cell RNA sequencing(scRNA-seq) to analyze cell-type specific changes in AS3MTd2d3-Tg mice as a schizophrenia model. To analyze using scRNAseq with E15.5, isocortex of AS3MT-Tg mice are dissected, digested with trypsin, and pipetted with Pasteur pipette to dissociate cells. To analyze using snRNAseq with P60, isocortex of AS3MT-Tg mice are applied to singulator to extract nuclei from cells.