<b>FLUID SHEAR STRESS MODULATES ENDOCYTIC PATHWAYS AND JUNCTIONAL TARGETING OF TUMOR-DERIVED EXTRACELLULAR VESICLES IN ENDOTHELIAL CELLS</b>

Here, we investigated TNBC sEV–endothelial interactions using combined in silico and in vitro approaches. Human umbilical vein endothelial cells (HUVECs) were cultured under static or FSS conditions (20 dyn/cm²), followed by proteomic profiling and protein–protein interaction analyses with sEV proteomes. FSS downregulated proliferation- and angiogenesis-associated proteins while upregulating adhesion and cytoskeletal regulators assessed by proteomics. Network analysis identified clathrin- and caveolin-mediated endocytosis (CME and CavME), integrins, and early endosomes as central mediators of sEV uptake.