The Induction of Trained Immunity in the Pancreas Incites Anti-tumor Activity to Control Pancreatic Cancer Progression

Following treatment with WGP, we have observed an influx of immune cell populations into the pancreas over time. These cells have increased anti-tumor activities, which results in protection against pancreatic cancer growth. The study objective is to better understand the cell types involved in this process and the temporal nature of this influx. It is known that the immune influx peaks at day 7, so we are interested in the changes in the immune populations of the pancreas 3 days and 7 days following WGP treatment. To gain a more in depth analysis of the cell populations present in the pancreas after WGP treatment, a single cell RNA sequencing (scRNA-Seq) was performed on sorted CD45+ cells from PBS treated mice and mice treated with WGP 3 and 7 days prior. Overall design: Mice were treated with PBS or WGP 7 days prior and 3 days prior to euthanization. The pancreas of these mice were harvested, digested, and stained with Viability dye and an anti-CD45 fluorescent antibody. Cells were then run through a cell sorter, where only live CD45+ cells were acquired. Cells were washed with 400ug/mL BSA 2x and then counted.