Identification of miR-141 as a new regulator of epidermal homeostasis, in cooperation with miR-203

To identify putative novel specific targets of miR-141-3p, we overexpressed this miRNAs in primary keratinocytes using a synthetic mimic (pre-miR-141-3p) or a synthetic “negative” control mimic (pre-miR-ctrl). RNA samples were harvested 30 hours post-transfection and 3 independent experiments were carried out. 3 independent experiments performed in a one color design, corresponding to 2 conditions (pre-miR-ctrl and pre-miR-141-3p) for a total of 6 samples.