Genomic profiling of MondoA and H3K27ac in primary human skeletal myotubes. Genomic profiling of MondoA and H3K27ac in primary human skeletal myotubes

It is well known that insulin resistance in the obese state is associated with accumulation of neutral lipid in extra-adipose tissues. This phenomenon is particularly well-documented in skeletal muscle. The mechanistic underpinnings of this linkage are not fully understood. To define direct MondoA target genes, we performed MondoA ChiP sequencing in human myotubes. Overall design: ChiP seq was performed in differentiated primary human skeletal myotubes. These cells were transfected with siRNA of control or MondoA. Two biological replicates for each condition were included.