Relative V2R/Rluc8 expression in live cells.
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HEK293FT or COS7 cells were transfected with the indicated V2R/Rluc8 constructs together with β-arrestin2/Venus. Luminescence was measured in triplicate wells after 2 h pre-treatment with 30 mM EnduRen, as part of the extended BRET kinetic assays (Figure 3). Data shown are the mean ± SEM of 3–7 independent experiments. RLU = relative light units. Luminescence intensity in cells not expressing Rluc8 was 30–150 RLU.
创建时间：
2015-12-02
