Legumain dependent gene signatures in stromal progenitors

LGMN has been shown to regulate cell fate decission of stromal progenitors of the bone. To understabnd the underlying mechanisms and gene signatures that are responsive to elevated or diminished levels of LGMN we performed RNA-seq analysis on stable cell lines with diminished (LGMN knockdown) or elevated (LGMN overexpression) levels of LGMN at the progenitor level. Genes responsive to LGMN levels show invers dynamics during osteoblast and adipocyte differentiation. Stable cell lines were generated from human stromal progenitor cells of bone marrow origin (BM-hMSC-TERT4 cells) with overexpression or knockdown of the asparaginyl endopeptidase (AEP) legumain (LGMN). RNA-seq was performed on 3 replicates of cells with LGMN overexpression (T4_LGMN) and control (T4_pBabe) as well as LGMN knockdown (T4_shLGMN) and control (T4_shCtrl).