CSF1R mediates effects of miR-34a loss during intestinal tumorigenesis in ApcMin mice (tumor organoid)

To address the in vivo relevance of CSF1R regulation by miR-34a during intestinal tumor formation, we generated ApcMin/+ mice with intestinal-epithelial cell (IEC)-specific deletions of the Mir34a and/or Csf1r genes. The mRNA expression profiles of tumor organoids derived ffrom intestinal adenomas with and without functional miR-34a and/or Csf1r were compared. Overall design: Mice were sacrificed at 18-20 weeks of age. Tumor organoids were grown for 3-4 passages. 3 tumor organoid RNA samples were obtained for each genotype; each tumor organoid RNA sample represented a pool of 3-5 tumors isolated from the same mouse.