CD8+ T lymphocytes in hypopigmented mycosis fungoides: malignant cells or reactive clone?

We used targeted single cell RNA sequencing using a gene set composed of the BD Rhapsody Human Immune Response Panel, TCR panel, and a custom panel of 61 malignancy-associated genes and T/B cell receptor sequencing to characterize single cell populations in hypopigmented mycosis fungoides (HMF). Using a reference mapping model built on publically deposited classic mycosis fungoides single cell RNA sequencing datasets, we identified a predicted malignant cell population independent from the clonal T cell population in HMF. Sequencing of skin cells from hypopigmented patches from one patient collected as three punch biopsies. Raw data not provided due to patient privacy concerns.