Transcriptional dissection of lateral root primordium cells

To specifically profile early stages of lateral root formation we used the marker pHB53:NLS-3xmCherry, which is only expressed in lateral root primordium cells. We isolated ~2,000 lateral root primordium cells from stages I to IV through Fluorescent Activated Cell Sorting, and profiled the transcriptome of 573 of these cells. As a LR primordium has ~6-10 cells at stage I and ~30-40 cells at stage IV, the number of sequenced cells would approximately cover 5-6 fold the total number of cells in the stages profiled. Overall design: Single-cell RNAseq of lateral root primordium cells at stages I-IV.