Competitive binding of ginsenosides to the guinea pig hepatic cytosolic AHR.

Guinea pig hepatic cytosol was incubated with 2 nM [3H]-TCDD in the absence or presence of 200 nM TCDF or the indicated ginsenoside for 2 hours at 20°C and [3H]-TCDD specific binding was determined using the hydroxyapatite binding assay as described in the Materials and Methods section. a Values were expressed as a percent of the total [3H]-TCDD specific binding and represented the mean ± SD of triplicate determinations. b Values represented that the amount of [3H]-TCDD specific binding was significantly displaced by the competitors at pt-test.