Viral load using TaqMan RT-PCR in ferrets infected with virus, A/VN/1203/04 (fg, mean±SD).

Note:a)Known concentrations of A/VN/1203/04 viral RNA (serially diluted: 108 to 10 fg) were used as templates and quantitative RT-PCR performed to generate a standard curve. 25∼30 mg of tissue from each organ was used for extraction of viral RNA and dissolved in 50 µl. 3 µl of the vRNA was used as template to perform real-time PCR. Each value represents the average concentration of six reactions based on the standard curve.b)indicates value for 1 animal.