HSV-1 infection induces a delay of promoter-proximal pausing linked to a shift of H2A.Z-containing +1 nucleosomes (ATAC-seq)

We investigated transposase accessible chromatin using ATAC-seq in mock and HSV-1 strain 17 infection and infection with several HSV-1 mutants. We used different HSV-1 mutants that lack expression of certain IE genes or VHS and examined the opening of chromatin downstream of transcription termination sites of affected genes. Two biological replicates (four replicates for delta-ICP22) of cells infected with different HSV-1 mutants at MOI 10 were prepared. ATAC-seq data for mock, WT strain 17 (+/- PAA) and delta-ICP0, delta-ICP22, delta-ICP7 and delta-vhs can also be found under accession GSE185234.