DNA sequences of transgenes detected via environmental DNA (raw ABI files, processed FASTA files, and reference alignments)

We demonstrate that simple, non-invasive environmental DNA (eDNA) methods
can detect transgenes of genetically modified (GM) animals from
terrestrial and aquatic sources in invertebrate and vertebrate systems. We
detected transgenic fragments between 82-234 bp through targeted PCR
amplification of environmental DNA extracted from food media of GM fruit
flies (Drosophila melanogaster), feces, urine, and saliva of GM laboratory
mice (Mus musculus), and aquarium water of GM tetra fish (Gymnocorymbus
ternetzi). With rapidly growing accessibility of genome-editing
technologies such as CRISPR, the prevalence and diversity of GM animals
will increase dramatically. GM animals have already been released into the
wild with more releases planned in the future. eDNA methods have the
potential to address the critical need for sensitive, accurate, and
cost-effective detection and monitoring of GM animals and their transgenes
in nature.