Joint-Structural Biology Group (JSBG) BAG proposal for ID29 beamline

The aim of the JSBG BAG proposal for ID29 beamline is to develop new methods for time-resolved serial crystallography experiments at 4th generation source and to study time dependent structural conformation of biological macromolecules that are studies at the ESRF-EMBL-GR Joint Structural Biology Group. Method developments include the study of radiation damage at high flux densities and with partially polychromatic beam (1% and 0.3%), the implementation and development of various sample delivery methods - mixing in capillary, microfluidics, tape-drive and fixed targets. This further includes development of GPU-based indexing algorithm, data reduction software, and develop new data collection strategies for pump-probe SSX experiments integrating nanosecond laser. Biological systems include the study of photoactivatable proteins, enzymatic systems through mixing experiments and multi-temperature jump experiments to study protein dynamics.