Summary of expression levels and allele specificity in human PBL.

Cluster/Locus - the cluster where the transcripts analysed (Transcripts in cluster & accession) are located. UCSC SNP annotation - the expressed polymorphism analysed for the allelic expression data. Methylated DMR - the differentially methylated regions associated with each cluster and the parental origin of the methylated allele,
*DMR identified in previously published work from other laboratories (see Table S1 for references); Prom  =  promoter; MAT/PAT  =  maternal/paternal allele methylated; G/S  =  DMR established in the germline/post fertilisation. P of O – the parental origin of the expressed allele
**established in previously published work from other laboratories – extensive references available for each gene @ http://igc.otago.ac.nz/table.html. Mat/Pat  =  maternal/paternal allele expressed. Mean ΔCT 1d.p. - the average of ΔCT values of PCL samples A and B for each gene to one decimal place. Generally ΔCT values above 9 ( = 2∧-dCT of 0.001) could be deteced by non-quantitative (nq) RT-PCR. U  =  below nq RT-PCR detection. The separate Q-PCR expression data from each individual are shown in Table S1. QPCR relative exp - the expression of these genes relative to the other tissues examined in Fig. S1. Pl  =  Placenta; Li  =  Liver; Br  =  brain; BM  =  bone marrow. Genes which were most highly expressed in PBL compared to fetal tissues are denoted ‘Highest’; in all cases except for SNRPN, these genes are biallelically expressed. RT-PCR - the number of nq RT-PCR cycles required to generate a product that could be sequenced reliably. The fractions in the Allelic Expression column indicate the number of biallelic or monoallelic samples there were of the total that were heterozygous (i.e. informative) for each SNP. The number of informative samples represents the total found following genotyping of 50 individuals., M/B  =  mono/biallelic. The complete data set and references, (data from humans and mice) can be found in Table S1.