Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Ahi1-/- mouse embryonic fibroblasts cells Transcriptomes

We report the application of RNA sequencing technology for high-throughput profiling of gene expression in mammalian cells. By sequencing from mouse embryonic fibroblasts RNA, we generated genome-wide gene expression maps of mouse embryonic fibroblasts cells. We found a significant difference of relatived gene in Ahi1+/+and Ahi1-/- cells in the basal state. These findings help to understand more functions and roles of the Ahi1 gene. Mouse embryonic fibroblasts cell mRNA profiles of wild type (WT) and Ahi1-/- mice