Time-course RNA-seq data for the cold response in Arabidopsis thaliana

To analyse changes in expression and alternative splicing in response to low temperatures, Arabidopsis thaliana (Col-0) plants were transferred from 20C to 4C and samples harvested every 3 hours for the last day at 20C, the first day at 4C and the fourth day at 4C giving 26 different time-points. The experiment was repeated three times independently giving 78 libraries, each of which were sequenced (Illumina paired-end non-stranded sequencing) on three different lanes generating 468 fastq files. The data is being analysed for differential gene expression and differential alternative splicing to show the important contribution of alternative splicing to re-programming of expression in response to cold stress.