BestKeeper results obtained for adipose tissue-derived mesenchymal stromal cells.

The last column (BK, n = 4) shows the parameters obtained after combining all stably expressed genes (B2M, RPL13A, HPRT1 and GAPDH), so they can be use as a single normalization factor. n: number of samples; BK: BestKeeper; GM [Cq]: geometric mean of Cq; AM [Cq]: arithmetic mean of Cq; Min [Cq]: minimum value of Cq; Max [Cq]: maximum value of Cq; SD [± Cq]: standard deviation of the Cq; CV [% Cq]: coefficient of variation expressed as a percentage on the Cq level; Min [x-fold]: minimum value of expression levels expressed as an absolute x-fold; Max [x-fold]: maximum value of expression levels expressed as an absolute x-fold; SD [± x-fold]: standard deviation of the absolute regulation coefficients. SD value obtained for ACTB (2.33) is higher than cutoff (1.0), so ACTB was defined as an unstably expressed gene and therefore was not considered for BK (n = 4) calculations.