Table_9_Seed Coat Pattern QTL and Development in Cowpea (Vigna unguiculata [L.] Walp.).csv

The appearance of the seed is an important aspect of consumer preference for cowpea (Vigna unguiculata [L.] Walp.). Seed coat pattern in cowpea has been a subject of study for over a century. This study makes use of newly available resources, including mapping populations, a reference genome and additional genome assemblies, and a high-density single nucleotide polymorphism genotyping platform, to map various seed coat pattern traits to three loci, concurrent with the Color Factor (C), Watson (W), and Holstein (H) factors identified previously. Several gene models encoding proteins involved in regulating the later stages of the flavonoid biosynthesis pathway have been identified as candidate genes, including a basic helix–loop–helix gene (Vigun07g110700) for the C locus, a WD-repeat gene (Vigun09g139900) for the W locus and an E3 ubiquitin ligase gene (Vigun10g163900) for the H locus. A model of seed coat development, consisting of six distinct stages, is described to explain some of the observed pattern phenotypes.

豆科植物豇豆（Vigna unguiculata [L.] Walp.）的种子外观是消费者对其偏好中的一个关键因素。豇豆种皮图案的研究已持续超过一个世纪。本研究利用新近可获得的资源，包括种群定位、参考基因组以及额外的基因组组装，以及高密度的单核苷酸多态性基因分型平台，将多种种皮图案性状映射至三个基因座，与先前确定的色素因子（C）、沃森因子（W）和荷斯坦因子（H）相一致。识别出多个编码参与调节黄酮类生物合成途径后期阶段的蛋白质的基因模型作为候选基因，包括C基因座的螺旋-环-螺旋基因（Vigun07g110700）、W基因座的WD重复基因（Vigun09g139900）以及H基因座的E3泛素连接酶基因（Vigun10g163900）。此外，还描述了一个由六个不同阶段组成的种皮发育模型，用以解释部分观察到的图案表型。