Gene expression profiling in lung and breast cancer cells treated by Bloom-specific siRNAs

A549 and MDA-MB-231 cells were transfected with the Bloom-specific siRNA. Total RNA was extracted using Trizol at 48 hours after transfection. RNA-SEQ was carried out to profile the gene expression in both culture conditions. The goal of this experiment was to profie the genes that are regulated by Bloom (BLM RecQ like helicase) in cancer cells. Gene expression was probed globally using RNA-SEQ.