Juvenile boars and testicular micro RNA

Data represent micro RNA concentrations in testicular tissue at pivotal points in Sertoli cell proliferation. Three in vivo pig models that stimulate Sertoli cell proliferation in vivo were evaluated. These three models were hemicastration on day 8, daily treatment with the androgen receptor blocker, flutamide, and weekly treatment with the aromatase inhibitor, letrozole. Time points were chosen to provide data on levels near the beginning of a demonstrable treatment effect Treatments Sertoli cell response and tissue and circulating hormone levels are described more completely in: Berger, T., Conley, A., 2014. Reduced endogenous estrogen and hemicastration interact synergistically to increase porcine Sertoli cell proliferation. Biol Reprod 90, 114; Berger, T., Sidhu, P., Tang, S., Kucera, H., 2019. Are testicular cortisol and WISP2 involved in estrogen-regulated Sertoli cell proliferation? Anim Reprod Sci 207, 44-51; and Legacki, E., Conley, A.J., Nitta-Oda, B.J., Berger, T., 2015. Porcine Sertoli cell proliferation after androgen receptor inactivation. Biol Reprod 92, 93. In each model, one littermate was treated and the remaining littermate served as the control. Testicular tissue was flash-frozen on dry ice. The miRNA was analyzed using miRCURY assay kits from Qiagen (Germantown, MD,USA) after isolation of mRNA using Qiazol cDNA synthesis as described in the kits. The micro RNA data are expressed as the delta Ct for the specific micro RNA relative to the reference micro RNA, miR-103 for each littermate. Samples within the four different data sets were potentially analyzed on different days with different lots of primer, which limits the value of comparisons between datasets.