RUS-refDB

RUS-refDB is a collection of protein sequences from rumen associated microorganisms, and the main purpose was to utilize it as a database for a rumen metaproteomic dataset. This comprises MAGs from metagenome recovered from rumen-incubated switchgrass enrichments (Hess et al. doi:10.1126/science.1200387 and Parks et al. doi: 10.1038/s41564-017-0012-7). We further included 11 genomes from GeneBank and the Hungate1000 project: Bacillus licheniformis strain, Butyrivibrio fibrisolvens, Prevotella ruminicola (2 strains), P. brevis, P. bryantii strain, Ruminococcus albus strain, R. flavefaciens (4 strains), Fibrobacter succinogenes and Methanobrevibacter ruminantium and the genomes of the five cultivated anaerobic fungi available at the time (~2018); Anaeromyces robustus, Neocallimastix californiae G1, Orpinomyces sp., Piromyces sp. E2 and Piromyces finnis, downloaded from MycoCosm (https://mycocosm.jgi.doe.gov). Viral scaffolds were also included in the database.