Expression data from rat dorsal root ganglion after chronic compression

Neuropathic pain (NP) is caused by primary or secondary impairment of the peripheral or central nervous systems. Its etiology is complex and involves abnormal patterns of gene expression and pathway activation. Using bioinformatic analysis, we aimed to identify NP-associated changes in genes and pathways in L4 and L5 dorsal root ganglia (DRG) in a rat model of NP induced by chronic compression of the DRG (CCD). All rats were divided into the sham and CCD groups randomly. Rats in CCD groups were anesthetized, followed by the implantation of two stainless steel rods in the intervertebral foramina between L4 and L5. Rats in the sham-operated group underwent the same procedure without steel rod insertion. DRG were harvested on the 7th day post-surgery. Total RNA was extracted and purified with a RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instruction. The quality and amount of RNA were measured using NanoDrop 2000 (Thermo, USA). mRNA expression profiling was performed using Affymetrix Clariom D Rat array (Affymetrix; Thermo Fisher Scientific, Inc), which contains 46,800 gene-level probe sets.