Bcl11b prevents catastrophic autoimmunity by controlling multiple aspects of regulatory T-cell gene expression program [ATAC-seq]

In order to investigate whether Bcl11b mediates any chormatin accebility in Treg cell, we performed ATAC-Seq from Bcl11b sufficient (WT) and Bcl11b deficient Treg(KO) cells.We used 100,000 cells for each experiment. Overall design: Genome wide occupancy profiling followed by high throughput sequencing for each conditions (3 replicates for Bcl11b WT Treg, 3 replicates for Bcl11b KO Treg) was done using Illumina HiSeq 2500.