Identification of Substrates of MBL Associated Serine Protease-1 (MASP-1) from Human Plasma using N-terminomics Strategy

Human blood was drawn from healthy adult volunteers after taking their informed consent. The study was approved by the institutional ethics committee, Ref. No. All India Institute of Medical sciences (AIIMS) /Patna/Institutional Ethics Committee/2019/413. All the experimental work was done in accordance with the relevant guidelines and regulations. Acid-citrate-dextrose (ACD) was used as an anticoagulant (6 vol. of blood to 1 vol. of ACD). Platelet-rich plasma was obtained by centrifuging the ACD blood at 200 g for 15 min. This was followed by plasma depletion of albumin and Immunoglobulin G (IgG) using antibody-based Abundant Protein Depletion Spin Columns (Pierce, Thermo Fisher, MA, USA). The antibodies were specific to the Fc domain of immunoglobulins.