4AB_7D_S8_Luciferase assay_heterochronic_mutants

Luciferase assay data used to quantify the duration of larval stages of animals of the indicated genotypes. The file contains three sheets: Raw data: Contains the raw values measured by the luminometer (Berthold Technologies, Centro XS3 LB 960). Luminescence was measured every 10 min for 0.5 seconds. The luminometer was placed in a temperature-controlled incubator set to 18.5°C, which translated to an effective temperature of 22.5°C experienced by the animals inside the luminometer. Plate design: Position of animals of indicated genotypes in the 384-well luminometer plate. In let-7(0), lin-46(0); let-7(0) and the triple mutant animals, only wells containing unbalanced animals were labeled and considered for the analysis. Annotated data: List of animals of indicated genotypes with the times of hatch, molt entry and exit and calculated durations of molt, intermolts and larval stages in hours. Animals excluded from the analysis are indicated by "0" in the "is_valid" column. WT HW1993 xeSi312[eft-3p::luc::gfp::unc-54 3'UTR, unc-119(+)] IV lin-46(0) HW3505 xeSi312 [eft-3p::luc::gfp::unc-54 3'UTR, unc-119(+)] IV; lin-46(ma385) V let-7(0) HW2791 xeSi312 [eft-3p::luc::gfp::unc-54 3’UTR, unc-119(+)] IV; let-7(xe150) X; xeEx365 [let-7p::let-7::SL1_operon_GFP, unc-119 (+); Prab-3::mCherry; Pmyo-2::mCherry; Pmyo-3::mCherry] lin-28(0); let-7(0) HW2793 lin-28(xe192) I; xeSi312 [eft-3p::luc::gfp::unc-54 3’UTR, unc-119(+)] IV; let-7(xe150) X lin-28(0); lin-46(0) HW3504 lin-28(xe192) I; xeSi312 [eft-3p::luc::gfp::unc-54 3'UTR, unc-119(+)] IV; lin-46(ma385) V lin-46(0); let-7(0) HW3544 xeSi312 [eft-3p::luc::gfp::unc-54 3’UTR, unc-119(+)] IV; lin-46(ma385) V; let-7(xe150) X; xeEx365 [let-7p::let-7::SL1_operon_GFP, unc-119 (+); Prab-3::mCherry; Pmyo-2::mCherry; Pmyo-3::mCherry] lin-28(0) HW2706 lin-28(xe192) I; xeSi312 [eft-3p::luc::gfp::unc-54 3’UTR, unc-119(+)] IV triple mut HW3543 lin-28(xe192) I; xeSi312 [eft-3p::luc::gfp::unc-54 3’UTR, unc-119(+)] IV; lin-46(ma385) V; let-7(xe150) X; xeEx365 [let-7p::let-7::SL1_operon_GFP, unc-119 (+); Prab-3::mCherry; Pmyo-2::mCherry; Pmyo-3::mCherry]