Arginine dependency is a therapeutically exploitable vulnerability in chronic myeloid leukaemic stem cells

We tested the effect of arginine depleting enzyme BCT-100 (24 hours) on primary normal and CML cells (100ng/mL for primary) as well as the wildtype or ASS1 knock-out K562 cell lines (1000ng/mL for cell lines) Cells were cultured in Plasmax Primary groups were Normal, Normal+bct, CML, CML+BCT Cell line groups were WT, WT+bct, ASS1KO, ASS1KO+BCT Overall design: Comparative gene expression profiling analysis of RNA-seq data of primary and cell lines Samples were profiled across 2 runs on sequencer. Data was merged to give 20 million reads/sample