Hepatic iNKT cells facilitate colorectal cancer metastasis by inducing a fibrotic niche in the liver

To better understand how disseminated cancer cells activate hepatic iNKT cells, we performed single-cell RNA sequencing of NKT cells sorted from livers 24 hours after intrasplenic injection of PBS or APTKA organoids Overall design: Hepatic NKT cells (single, live, CD45+, CD115-, F4/80-, CD19-, Ly6G-, CXCR6+, CD8-, NK1.1+, NKp46- cells) were isolated by fluorescence-activated cell sorting (FACS) 24 hours after intrasplenic injection of PBS or APTKA organoids. 5 mice per conditions were used. Cells from each sample were labeled using the BD Single-Cell Multiplexing Kit and pooled according to their respective conditions.