Summary of amino-acid sequence differences between the vBACDV1 and the panel of DENV infectious cDNA clones containing the selected subset of substitutions in the E and/or NS3 proteins.

aPosition of codon change within the individual protein-encoding region.bParental strain from which the neuroadapted strains were derived (AF226686.2) [45]–[46].cNeuroadapted strain (AF226686.1).dNeuroadapted strain (EF122231.1).evBACDV1 differs from the original BR/90 GenBank-deposited sequence (AF226685.2) by only 11 synonymous nucleotide substitutions; both BR/90 and vBACDV1 are identical to the parental virus used for previous neuroadaptation studies (strain FGA/89; [45]–[46]) for the 5 codons shown in this table (see Table S1).*Mutations studied.